Journal: Frontiers in Endocrinology
Article Title: Effects of Total Flavonoids of Epimedium on Bone Marrow Adipose Tissue in Ovariectomized Rats
doi: 10.3389/fendo.2022.900816
Figure Lengend Snippet: Representative microscopic features of PPARγ2 and FABP4 expression in bone marrow (200x magnification). Quantification of PPARγ2 expression by immunofluorescence (A) and FABP4 expression by immunohistochemistry (B) in the bone marrow showed a significant increase in PPARγ2 and FABP4 protein expression in proximal tibiae of OVX rats, which were restored to normal levels after TFE treatment. For PPARγ2, the positively staining pixels were presented as a percentage of the total pixels (C) ; for FABP4, semi-quantification is expressed as the relative integrated intensity of intramarrow staining (D) . FABP4, fatty acid-binding protein 4; PPARγ2, peroxisome proliferator-activated receptor gamma-2; OVX, ovariectomy; Sham, sham-operation; TFE, total flavonoids of Epimedium; Ve, vehicle. Data are shown as mean ± SD (Sham, n = 11; OVX, n = 10; TFE, n = 11). ** P <0.001 between groups analyzed by One-way ANOVA with Bonferroni-correction.
Article Snippet: The rats were randomly divided into three groups with 12 per group :(1) sham-operation group (Sham), (2) ovariectomized group (OVX), and (3) OVX treated with TFE group (TFE, 191 mg/kg body weight/day) (Kanion Sunshine Pharmaceutical Co., Ltd., Jiangsu, China).
Techniques: Expressing, Immunofluorescence, Immunohistochemistry, Staining, Binding Assay